Lipases are enzymes that break down fats into fatty acids and glycerol or monoglycerides or other alcohols through a process called hydrolysis. Hydrolysis is the chemical breakdown of a compound due to reaction with water. In the case of fats, hydrolysis breaks down the ester bonds that hold the fatty acids to the glycerol molecule. This process results in the formation of fatty acids and glycerol .

Lipases are activated by various hormones such as glucagon, epinephrine and growth hormone. In the fat cell, other types of lipases work to break down fats into fatty acids and glycerol. Lipid metabolism begins in the intestine where ingested triglycerides are broken down into free fatty acids and a monoglyceride molecule by pancreatic lipases, enzymes that break down fats after they are emulsified by bile salts.

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